Genes encoding the seven precursor constructs, four natural variants, and three DRMs were synthesized and cloned in pET11a vector between Nde1 and BamH1 sites. The protocol described here permits the isolation and detailed study of PR precursors that were previously extremely difficult to obtain due to their intrinsic autoprocessing during expression. Old Moms Tgp Masha is a pretty young slut playing. This pregnant woman fucks with her boyfriend. Autoprocessing of HIV-1 protease is tightly coupled to protein folding. Initial cleavage of the human immunodeficiency virus type 1 GagPol precursor by its activated protease occurs by an intramolecular mechanism.
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